Protein Ladder

MOLEQULE-ON Blue Protein Marker is a three-colour standard containing 12 pre-stained proteins covering a broad molecular weight range of 10 to 245 kDa. When separated on SDS-PAGE using Tris-glycine buffer, the proteins are covalently coupled with a blue chromophore, with the exception of two reference bands at ~25 kDa and ~90 kDa, which are coupled with green and red chromophores respectively for easy identification. The marker is supplied ready-to-use in a loading buffer and produces sharp, well-defined bands.

The marker is dissolved in a buffer composed of 20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol. Its intended applications include monitoring of protein migration during SDS-polyacrylamide gel electrophoresis, monitoring of protein transfer onto membranes during Western blotting, and sizing of proteins on SDS-polyacrylamide gels and Western blots.