Porcine Transforming Growth Factor Beta 1 ELISA Kit

Antibodies.com supplies a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) intended for the in vitro quantitative determination of porcine Transforming Growth Factor Beta 1 (TGF beta 1) in serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids. The assay is run on a pre-coated microplate and uses a biotinylated anti-TGF beta 1 detection antibody with an HRP-avidin conjugate and TMB substrate; absorbance is read at 450 nm on a colorimetric microplate reader.

According to the manufacturer, the kit has a product range of 31.2–2000 pg/ml and a sensitivity of <10.5 pg/ml, with a total assay time of 4 h 30 min. Reported spike-and-recovery values are 80–102% for serum (average 91%, n=5), 81–100% for EDTA plasma (average 90%, n=5) and 80–89% for heparin plasma (average 84%, n=5), and dilution linearity is documented across 1:2 to 1:16 in the same matrices. Kit components should be stored at +4°C or −20°C as instructed and the kit should not be used past its expiration date.