Porcine Tissue Inhibitors Of Metalloproteinase 4 ELISA Kit

Antibodies.com offers a sandwich ELISA kit for the in vitro quantitative determination of porcine Tissue Inhibitor of Metalloproteinase 4 (TIMP4) in serum, plasma, tissue homogenates, or other biological fluids. The assay uses a pre-coated microplate arranged as 12 x 8 well strips, a biotinylated anti-TIMP4 detection antibody, and an HRP-avidin conjugate. Signal is generated using TMB substrate, and the resulting absorbance is read at 450 nm on a colorimetric microplate reader.

The reported sensitivity is 0.068 ng/ml and the assay range is 0.156–10 ng/ml. The total assay time is 4 hours 30 minutes. Spike-and-recovery testing showed average recoveries of 91% in serum, 90% in EDTA plasma, and 84% in heparin plasma. Linearity was demonstrated at sample dilutions of 1:2, 1:4, 1:8, and 1:16 across serum, EDTA plasma, and heparin plasma.

Provided components include a pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and adhesive plate sealers, with individual storage temperatures of either +4°C or -20°C. Kit components should not be used past the stated expiration date.