Porcine Regulated On Activation In Normal T-Cell Expressed And Secreted ELISA Kit

A quantitative sandwich ELISA from Antibodies.com for the in vitro measurement of porcine RANTES (Regulated On Activation In Normal T-Cell Expressed And Secreted) in serum, plasma or other biological fluids. The kit employs a sandwich immunoassay format using a pre-coated microplate, a biotinylated anti-RANTES detection antibody, and an HRP–avidin conjugate with TMB substrate, producing a yellow product whose absorbance is read at 450 nm.

Reported assay sensitivity is 1.15 ng/ml with a working range of 3.12-200 ng/ml, and the protocol runs in approximately 4h 30m. Spike-and-recovery performance reported by the manufacturer is 80-102% (average 91%) in serum, 81-100% (average 90%) in EDTA plasma, and 80-89% (average 84%) in heparin plasma across undiluted samples (n=5), with linearity demonstrated on dilution.