Porcine Interleukin 1 Beta ELISA Kit

The brand Antibodies.com offers a sandwich ELISA kit for the in vitro quantitative determination of porcine IL-1 beta in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips and employs the sandwich enzyme immunoassay technique, with detection performed on a colorimetric microplate reader at 450 nm.

An antibody specific for IL-1 beta is pre-coated onto the plate; standards and samples are added, followed by washing and incubation with a biotinylated anti-IL-1 beta antibody. HRP-Avidin conjugate is added, and TMB substrate generates a blue-coloured product that turns yellow upon addition of acidic stop solution. The density of yellow is proportional to the amount of IL-1 beta captured in each well. The product range is 62.5-4000 pg/ml, with a sensitivity of <26.2 pg/ml, and the total assay time is 4 hours 30 minutes.

Manufacturer recovery data show average recovery of 91% for serum (n=5, range 80-102%), 90% for EDTA plasma (n=5, range 81-100%), and 84% for heparin plasma (n=5, range 80-89%). Linearity assessed at dilutions of 1:2, 1:4, 1:8, and 1:16 gave serum (n=5) recoveries of 87-91%, 87-107%, 74-101%, and 92-97% respectively; EDTA plasma (n=5) at 90-105%, 84-101%, 90-101%, and 79-108%; and heparin plasma (n=5) at 84-95%, 92-105%, 82-105%, and 89-91%. Kit components include a pre-coated 96-well microplate, lyophilized standard, detection solutions A and B, wash buffer (30X), sample dilution buffer, TMB substrate, stop solution, and adhesive plate sealers. Storage instructions call for components to be kept at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.