Porcine High Mobility Group Protein 1 ELISA Kit

Antibodies.com distributes a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) intended for the in vitro quantitative determination of porcine High Mobility Group Protein 1 (HMGB1) in serum, plasma and other biological fluids. The Porcine HMGB1 ELISA Kit employs a pre-coated 96-well microplate arranged as 12 x 8 well strips and is designed to be read on a colorimetric microplate reader.

Reported performance characteristics include a sensitivity of 28.3 pg/ml and an assay range of 62.5–4000 pg/ml, with a total assay time of approximately 4 hours 30 minutes. Detection relies on a Biotinylated Anti-HMGB1 Antibody binding to captured HMGB1, followed by addition of HRP-Avidin conjugate and TMB substrate. The resulting colour change is read at 450 nm and quantified by reference to the standard curve. Kit components should be stored at +4°C or –20°C as indicated, and the product must not be used past its expiration date.

Provided spike-and-recovery data show averages of 91% in serum (range 80–102%), 90% in EDTA plasma (range 81–100%) and 84% in heparin plasma (range 80–89%). Linearity of dilution has been demonstrated across 1:2 to 1:16 dilutions for serum, EDTA plasma and heparin plasma samples (n = 5), supporting the kit's suitability for these specimen types.