Porcine Fibroblast Growth Factor 7 ELISA Kit

From Antibodies.com, this Porcine KGF/FGF7 (Fibroblast Growth Factor 7) ELISA Kit is a sandwich enzyme-linked immunosorbent assay designed for the in vitro quantitative determination of porcine KGF/FGF7 in serum, plasma, tissue homogenates, or other biological fluids. The assay employs a pre-coated 96-well microplate with HRP-Avidin conjugate detection and TMB substrate, with results read colorimetrically at 450 nm using a microplate reader. Total assay time is 4h 30m.

Reported performance includes a measuring range of 15.6-1000 pg/ml and a sensitivity of 5.9 pg/ml. Spiking recovery across n=5 samples averaged 91% for serum (range 80-102%), 90% for EDTA plasma (range 81-100%), and 84% for heparin plasma (range 80-89%). Dilution linearity at 1:2, 1:4, 1:8 and 1:16 dilutions returned ranges of 87-91%, 87-107%, 74-101% and 92-97% respectively for serum (n=5); 90-105%, 84-101%, 90-101% and 79-108% for EDTA plasma; and 84-95%, 92-105%, 82-105% and 89-91% for heparin plasma. Kit components are stored at +4°C or -20°C as instructed.