Porcine Apolipoprotein E ELISA Kit

From Antibodies.com, this Porcine Apolipoprotein E ELISA Kit is a sandwich enzyme-linked immunosorbent assay (sELISA) designed for the in vitro quantitative determination of porcine Apolipoprotein E in serum, plasma or other biological fluids. The assay employs a pre-coated 96-well microplate arranged as 12 x 8 well strips, with standards and test samples incubated alongside a Biotinylated Anti-Apolipoprotein E Antibody and an HRP-Avidin conjugate. A TMB substrate and acidic stop solution generate a yellow product whose absorbance is read at 450 nm, enabling quantification against the standard curve. The stated assay range is 1.25–80 ng/ml with a sensitivity of <0.56 ng/ml, and the total assay time is 4 h 30 m. Reported sample recovery averaged 91% in serum (range 80–102%), 90% in EDTA plasma (range 81–100%), and 84% in heparin plasma (range 80–89%). Dilution linearity was assessed at 1:2, 1:4, 1:8 and 1:16 across serum, EDTA plasma and heparin plasma matrices. Kit components are stored at +4 °C or –20 °C, and the assay is intended for use with a colorimetric microplate reader.