Porcine Alpha-Fetoprotein ELISA Kit

Each Antibodies.com Porcine Alpha-Fetoprotein ELISA Kit is supplied as a 48-test format containing a pre-coated microplate, lyophilized recombinant porcine AFP standard, biotinylated capture antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop solution. The quantitative assay measures Alpha-Fetoprotein in swine samples across a standard curve of 0.625 to 40 ng/mL with a minimum detectable dose of approximately 0.1 ng/mL, requiring only a 50 µL sample per well. Reconstituted standards should be aliquoted to limit freeze-thaw cycles, and unopened reagents are shipped refrigerated and protected from light. This reagent supports reproductive biology, fetal development, and hepatic injury research in swine, where AFP serves as a biomarker for hepatocellular damage, yolk-sac activity, and prenatal liver function. The kit is validated for porcine blood-derived fractions, cell culture supernatant, and tissue homogenate, with no significant cross-reactivity observed against albumin or transferrin. Intended for research use only.