Ms ACE2 ELISA Kit

STEMCELL Technologies offers a sandwich ELISA kit for the quantitative detection and measurement of mouse angiotensin converting enzyme 2 (ACE2) in biological fluids such as serum, plasma, cell culture supernatant, and tissue homogenates. ACE2 is described as a type I integral membrane protein that is highly expressed in capillary-rich organs including the lungs, heart, blood vessels, kidneys, liver, and gastrointestinal tract. The ACE2 receptor is the target of the SARS-CoV-2 Spike (S) glycoprotein, and the interaction that allows viral entry into the host cell makes ACE2 a potential target for antiviral therapeutics.

The assay uses strip plates pre-coated with capture antibodies specific for ACE2. Captured analyte is detected using a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase (SA-HRP) and the chromogenic enzyme substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the colored product is directly proportional to the concentration of ACE2 in the sample, which is determined by comparison to a serial dilution of the standard analyzed in parallel. The recommended standard curve dilutions range from 6 - 400 pg/mL, with a reportable assay range of 6.25 - 400 pg/mL. Absorbance is measured at 450 nm, with an optional correction wavelength of 540 nm or 570 nm. The kit may be shipped at room temperature (15 - 25°C) but should be stored at 2 - 8°C upon receipt.