MQ cDNA Synthesis Kit

Engineered by MOLEQULE-ON for research and laboratory cDNA synthesis applications, this kit overcomes the most challenging secondary RNA structures across a wide temperature range. It contains a next-generation engineered recombinant M-MLV reverse transcriptase featuring improved thermostability, processivity, robustness, and optimal cDNA yields, along with proprietary site mutations for reduced RNase H activity and an extended half-life.

Stability RT-qPCR data indicate the reverse transcriptase maintains performance at 37°C for 3 weeks (21 days) without significant alteration. In one-step RT-qPCR testing of the N1 gene at 100 copies per reaction, a mean Ct of 34.88 was recorded. The enzyme shows stable and superior performance across temperatures of 42–60°C, including up to 60°C, and has been demonstrated to synthesise cDNA from human bladder cancer cell line RNA templates up to 11 kb in length, with 8 kb and 11 kb bands visualised on a 2% agarose gel. Better efficiency and higher cDNA yields are reported at 55°C relative to other reverse transcriptases.

Kit components include MQ RScript Enzyme Mix, 2X Sharp Reaction Mix, Oligos d(T)20 Primer, Random Hexamer Primer, and Nuclease free Water. The kit is intended for use with total RNA, synthetic RNA transcripts, or poly(A)+ mRNA, with reactions assembled in 20 µl volumes. The procedure involves pre-mixing the RNA template, primer, and 2X Sharp Reaction Mix at 65°C for 5 minutes on ice for at least 1 minute, then incubating the assembled reaction at 55°C for 50 minutes for extension, followed by 70°C for 15 minutes for reverse transcriptase inactivation. The kit should be stored at -20°C under dark conditions.