Mouse VEGF Receptor 2 ELISA Kit

VEGFR-2 (also known as KDR or Flk-1) is the principal receptor tyrosine kinase that mediates VEGF-A-driven signaling and serves as a central regulator of angiogenesis, vascular permeability, and endothelial cell proliferation. The Mouse VEGF Receptor 2 ELISA Kit from Antibodies.com provides a quantitative immunoassay format for measuring VEGFR-2 concentrations in mouse-derived samples, supporting studies in tumor vascularization, cardiovascular disease models, wound healing research, and developmental angiogenesis.

The 96T configuration includes a pre-coated microplate, lyophilized recombinant mouse VEGFR-2 reference standards, a biotinylated detection antibody, streptavidin-HRP reagents, wash buffer, substrate, and stop solution. Researchers typically apply this assay to cell culture supernatant, tissue lysates, and other mouse biological matrices, generating a seven-point standard curve to interpolate unknown VEGFR-2 levels in experimental samples.

Storage instructions call for refrigerated handling of unopened components, with the standard and detection antibody aliquoted and stored as detailed in the manual. All reagents are designated for research use only and have not been validated for diagnostic procedures. Each kit provides sufficient reagents for one full plate, allowing the standard curve plus experimental and control wells to be run in a single experiment.