Mouse Vasorin ELISA Kit

From Antibodies.com, this sandwich ELISA is intended for the in vitro quantitative determination of mouse Vasorin in tissue homogenates or other biological fluids, with reactivity specified for mouse. The assay employs a pre-coated microplate and follows a sandwich enzyme immunoassay workflow: standards and samples are added to the wells, captured Vasorin is detected using a biotinylated anti-Vasorin antibody, and an HRP-Avidin conjugate is added before visualisation with TMB substrate. Absorbance is read at 450 nm in a colorimetric microplate reader.

Sensitivity is reported as <0.060 ng/ml, with a measuring range of 0.156–10 ng/ml, and a total assay time of 4 hours 30 minutes. Sample recovery has been evaluated in serum (80%–102%, average 91% across 5 samples), EDTA plasma (81%–100%, average 90% across 5 samples), and heparin plasma (80%–89%, average 84% across 5 samples). Dilution linearity has been tested at 1:2, 1:4, 1:8, and 1:16 across these sample types.

Kit components include lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and adhesive plate sealers, with storage temperatures specified per component between +4°C and −20°C.