Mouse Tumor Necrosis Factor-alpha ELISA Kit

This 192-test ELISA kit from Antibodies.com enables quantitative detection of mouse Tumor Necrosis Factor-alpha (TNF-α), a key pro-inflammatory cytokine implicated in immune signaling, apoptosis regulation, and acute-phase responses. TNF-α is a frequent biomarker in murine research models of inflammation, autoimmune disease, sepsis, and host-pathogen interaction.

The kit provides pre-coated microplate wells, capture and detection antibodies, recombinant mouse TNF-α standards, and assay buffers sufficient for 192 individual determinations. Researchers commonly apply the assay to cell culture supernatants, tissue homogenates, and serum collected from mouse models to monitor endogenous cytokine release in response to pharmacological or immunological stimuli.

Reactivity is specific to mouse TNF-α, supporting studies that compare findings across murine in vivo and in vitro systems. Each lot ships with a certificate of analysis outlining lot-specific validation data, and the protocol recommends appropriate dilution ranges for typical sample matrices encountered in mouse immunology workflows.

Research applications

• Cytokine profiling in mouse inflammation models
• Evaluation of anti-inflammatory compounds in murine disease systems
• Time-course studies of TNF-α release following immune challenge
• Phenotypic characterization of transgenic or knockout mouse lines
• Validation of cell-based assays measuring immune-cell activation

Storage conditions for individual components are specified on the certificate of analysis to preserve reagent activity through intended use.