Mouse Tryptophan Hydroxylase 1 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com enables the in vitro quantitative determination of mouse Tryptophan Hydroxylase 1 (TPH) in tissue homogenates, cell lysates, and other biological fluids. The assay uses the sandwich enzyme immunoassay technique, with a TPH-specific antibody pre-coated onto a 96-well microplate. Standards and samples are added, followed by a biotinylated anti-TPH detection antibody and an HRP-avidin conjugate; signal is generated using TMB substrate and quantified by reading the absorbance at 450 nm on a colorimetric microplate reader.

According to the manufacturer's specification, the kit has a product range of 0.156–10 ng/ml and a sensitivity of <0.054 ng/ml. The total assay time is 4 hours 30 minutes, and the pre-coated plate is configured as 12 x 8 well strips. Kit components are stored at +4°C or -20°C as instructed, and the reagents are described as ready-to-use.

Reported average recovery values for spiked samples were 91% in serum, 90% in EDTA plasma, and 84% in heparin plasma, with acceptable linearity demonstrated across dilutions of 1:2, 1:4, 1:8, and 1:16 in each of these matrices. The kit should not be used past its stated expiration date.