Mouse Troponin T Type 1, Slow Skeletal ELISA Kit

Manufactured for the quantitative determination of mouse Troponin T1/TNT, this sandwich ELISA kit from Antibodies.com is intended for in vitro measurement of the target in serum, plasma, or other biological fluids. The assay uses a pre-coated microplate format with biotinylated detection antibody and HRP-avidin/TMB substrate chemistry, with absorbance read at 450 nm on a colorimetric microplate reader.

According to the manufacturer's documentation, the assay has a sensitivity of 5.8 pg/ml and a working range of 15.6–1000 pg/ml, with a total assay time of 4 hours 30 minutes. Kit components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.

Reported validation data show average spike recovery of 91% in serum (range 80–102%, n=5), 90% in EDTA plasma (range 81–100%, n=5), and 84% in heparin plasma (range 80–89%, n=5), with dilution linearity demonstrated from 1:2 through 1:16 in all three sample types.