Mouse Transmembrane Protease, Serine 2 ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the in vitro quantitative determination of mouse Transmembrane Protease, Serine 2 (TMPRSS2) in serum, plasma, tissue homogenates, and other biological fluids. The assay uses a pre-coated microplate format and is read on a colorimetric microplate reader at 450 nm.

Reported specifications include a sensitivity of <0.113 ng/ml and an assay range of 0.312–20 ng/ml, with a total assay time of approximately 4 hours 30 minutes. Reactivity is limited to mouse samples. Recovery has been evaluated in serum, EDTA plasma, and heparin plasma across multiple sample dilutions.

The assay employs a sandwich immunoassay: standards and samples are added to wells pre-coated with anti-TMPRSS2 antibody, followed by incubation with a Biotinylated Anti-TMPRSS2 Antibody and an HRP-Avidin conjugate. TMB substrate is used for color development, the reaction is stopped with an acidic stop solution, and absorbance is read at 450 nm. Kit components should be stored at +4°C or −20°C as indicated, and the kit should not be used past its expiration date.