Mouse Transforming Growth Factor Beta 3 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com for the in vitro quantitative determination of mouse Transforming Growth Factor Beta 3 (TGF beta 3) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 × 8 well strips, with TGF beta 3 captured by an immobilized specific antibody and detected using a Biotinylated Anti-TGF beta 3 Antibody followed by an HRP-Avidin conjugate. Signal is generated with TMB substrate solution and read at 450 nm on a colorimetric microplate reader, with TGF beta 3 concentration calculated from the standard curve.

Reported performance characteristics include a product range of 15.6-1000 pg/ml and a sensitivity of <6.3 pg/ml. The total assay time is 4 hours 30 minutes, and kit components are stored at +4°C or -20°C as instructed in the protocol.

Average recovery values across sample matrices are 91% for serum (range 83-99%, n=5), 89% for EDTA plasma (range 80-99%, n=5), and 90% for Heparin plasma (range 82-99%, n=5). Dilution linearity has been evaluated at 1:2, 1:4, 1:8 and 1:16 for the same sample types.