Mouse Transforming Growth Factor Alpha ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay from Antibodies.com is designed for the quantitative measurement of mouse Transforming Growth Factor Alpha (TGF alpha) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The format employs a pre-coated microplate and a biotinylated anti-TGF alpha antibody with an HRP-Avidin conjugate, and the HRP enzymatic reaction is visualised with TMB substrate to produce a yellow colour read at 450 nm on a standard microplate reader.

The assay has a stated sensitivity of <5.5 pg/ml and a product range of 15.6-1000 pg/ml. Total assay time is 4 hours 30 minutes. Manufacturer recovery data report an average of 91% in serum (range 80%-102%), 90% in EDTA plasma (range 81%-100%) and 84% in heparin plasma (range 80%-89%), with dilution-linearity values supplied across 1:2, 1:4, 1:8 and 1:16 sample dilutions.

Supplied components include a pre-coated 96-well microplate, lyophilised standard (2 vials), Detection Solution A, Detection Solution B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and plate sealers. Components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date. The product is listed as having been cited in at least one published study.