Mouse Thrombospondin 1 ELISA Kit

This sandwich ELISA from Antibodies.com is designed for the in vitro quantitative determination of mouse Thrombospondin 1 in plasma, tissue homogenates, or other biological fluids. The assay uses a pre-coated 96-well microplate arranged in 12 x 8 well strips and follows a sandwich enzyme immunoassay workflow, employing a biotinylated anti-Thrombospondin 1 detection antibody, HRP-avidin conjugate, and TMB substrate. Absorbance is read at 450 nm using a colorimetric microplate reader, with analyte concentrations calculated against a standard curve.

The reported assay range is 15.6–1000 pg/ml with a sensitivity of <6.6 pg/ml, and the total assay time is 4 hours 30 minutes. Spike recovery ranges of 80–102% in serum, 81–100% in EDTA plasma, and 80–89% in heparin plasma have been observed. Dilution linearity assessed at 1:2, 1:4, 1:8, and 1:16 dilutions shows ranges spanning 74–107% for serum, 79–108% for EDTA plasma, and 82–105% for heparin plasma. Kit components should be stored at +4°C or –20°C as indicated on each component, and the kit should not be used past its expiration date.