Mouse Thrombin Activatable Fibrinolysis Inhibitor ELISA Kit

This Sandwich ELISA kit is offered by Antibodies.com under the Assays and Kits category for the mouse Thrombin Activatable Fibrinolysis Inhibitor target. The manufacturer's product specification, filed under Mouse Carboxypeptidase B2 ELISA Kit, describes a sandwich enzyme immunoassay on a pre-coated 96-well microplate arranged as 12 x 8 well strips, with plasma as the indicated sample type.

The detection is colorimetric: a biotinylated anti-target antibody binds the captured analyte, HRP-avidin is added, and TMB substrate produces a colour change read at 450 nm. Reported figures are a sensitivity of <0.32 ng/ml, an assay range of 0.781–50 ng/ml, and a total assay time of 4 hours 30 minutes.

Spiking-and-recovery data (n=5) give average values of 91% (range 80%–102%) in serum, 90% (81%–100%) in EDTA plasma, and 84% (80%–89%) in heparin plasma. Dilution linearity at 1:2, 1:4, 1:8 and 1:16 (n=5) was reported as serum 87%–107%, EDTA plasma 79%–108%, and heparin plasma 82%–105% across dilutions. Kit components are stored at +4°C or −20°C as directed and should not be used past the expiration date.