Mouse Taste Receptor Type 1 Member 2 ELISA Kit

From Antibodies.com, this sandwich ELISA kit enables quantitative in vitro determination of mouse GPCR TAS1R2 in tissue homogenates, cell lysates and other biological fluids. The assay uses a pre-coated 96-well microplate together with a biotinylated anti-GPCR TAS1R2 detection antibody, HRP-avidin conjugate and TMB substrate, producing a colourmetric readout measurable at 450 nm.

The stated assay range is 0.156-10 ng/ml with a sensitivity of <0.043 ng/ml, and the total assay time is 4h 30m. Reported average recoveries across the tested dilution series are 91% for serum, 91% for EDTA plasma and 84% for heparin plasma.

Kit components include a pre-coated microplate, lyophilised standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, with storage temperatures ranging from +4°C to -20°C depending on the component.