Mouse Sphingosine 1 Phosphate Lyase 1 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay from Antibodies.com, designed for the in vitro quantitative determination of mouse Sphingosine 1 Phosphate Lyase 1 (SGPL1) in tissue homogenates or other biological fluids. The assay uses the sandwich enzyme immunoassay technique on a pre-coated 96-well microplate (12 x 8 well strips), with standards and test samples bound by an immobilized SGPL1-specific antibody, followed by a Biotinylated Anti-SGPL1 Antibody, HRP-Avidin conjugate, and TMB substrate, read at 450 nm on a colorimetric microplate reader.

The reported product range is 0.156-10 ng/ml with a sensitivity of <0.055 ng/ml, and a total run time of 4h 30m. Kit components are stored at +4°C or -20°C as instructed.

Spike-and-recovery data are provided for serum (n=5, 80%-102%, mean 91%), EDTA plasma (n=5, 81%-100%, mean 90%), and heparin plasma (n=5, 80%-89%, mean 84%), with additional recovery values reported across 1:2 to 1:16 dilutions.