Mouse Solute Carrier Family 30 Member 7 ELISA Kit

From Antibodies.com, this Sandwich ELISA kit is designed for the in vitro quantitative determination of mouse ZnT7 (Solute Carrier Family 30 Member 7) in tissue homogenates or other biological fluids. The assay employs the sandwich enzyme immunoassay technique, with an antibody specific for ZnT7 pre-coated onto a 96-well microtiter plate arranged as 12 x 8 well strips. Standards and test samples are added to the wells, followed by a Biotinylated Anti-ZnT7 Antibody and an HRP-Avidin conjugate. TMB substrate solution is then used to visualise the HRP enzymatic reaction, producing a blue-coloured product that turns yellow upon addition of acidic stop solution. The density of yellow is proportional to the amount of ZnT7 captured in each well, and absorbance is read at 450 nm in a colorimetric microplate reader.

The stated assay range is 0.156-10 ng/ml with a sensitivity of 0.053 ng/ml, and the total assay time is 4h 30m. Components supplied include a Pre-Coated 96 Well Microplate, Lyopholized Standard, Detection Solution A, Detection Solution B, Wash Buffer (30X), Sample Dilution Buffer, TMB Substrate, Stop Solution, and Plate Sealers. Kit components should be stored at +4°C or -20°C as instructed and the kit should not be used past its expiration date.

The manufacturer provides recovery data for serum, EDTA plasma, and heparin plasma samples tested at dilutions from 1:2 through 1:16.