Mouse Solute Carrier Family 30, Member 3 ELISA Kit

From Antibodies.com, this sandwich ELISA is designed for the in vitro quantitative determination of mouse ZnT3 (Solute Carrier Family 30, Member 3) in tissue homogenates or other biological fluids [C0, S1]. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with a colourmetric detection format read at 450 nm in a microplate reader [S1].

The reported assay range is 0.312–20 ng/ml with a sensitivity of <0.114 ng/ml, and the total assay time is 4 hours 30 minutes [S1]. Kit components are stored at +4°C or -20°C as instructed, and the product should not be used past its expiration date [S1].

According to the manufacturer, the kit includes a pre-coated plate, lyophilised standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers [S1]. Reported sample recovery values include serum (average 91%, range 80–102%), EDTA plasma (average 91%, range 81–101%), and heparin plasma (average 84%, range 80–89%) across dilutions from 1:2 to 1:16 [S1].