Mouse Solute Carrier Family 30, Member 1 ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of mouse ZnT1 (Solute Carrier Family 30, Member 1) in tissue homogenates, cell lysates, and other biological fluids. The assay uses a pre-coated microplate format with a biotinylated anti-ZnT1 detection antibody and HRP-avidin conjugate; signal is developed with TMB substrate, stopped with acidic solution, and read as optical density at 450 nm on a colorimetric microplate reader.

Quantitative performance is characterised across an assay range of 0.156–10 ng/ml with a reported sensitivity of 0.054 ng/ml. The full protocol runs in approximately 4 hours 30 minutes. Recovery has been evaluated in serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, 1:8, and 1:16, with reported average recoveries of 91% (serum), 91% (EDTA plasma), and 84% (heparin plasma). Components are intended for storage at +4°C or −20°C as indicated, and the kit is not to be used past its stated expiration date.