Mouse Sirtuin 6 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com is designed for the in vitro quantitative determination of mouse Sirtuin 6 (SIRT6) in tissue homogenates, cell lysates, and other biological fluids. The assay uses a pre-coated microplate format, a biotinylated anti-SIRT6 detection antibody, an HRP-avidin conjugate, and a TMB substrate that yields a colour change read at 450 nm.

Reported sensitivity is 0.063 ng/ml and the assay range is 0.156–10 ng/ml, with a typical run time of approximately 4 hours 30 minutes. Kit components are stored at +4°C or -20°C as instructed, and the product is intended for research use only, not for diagnostic or therapeutic applications.

Manufacturer validation includes recovery testing in serum (range 80–102%, average 91%, n=5), EDTA plasma (81–100%, average 90%, n=5), and heparin plasma (80–89%, average 84%, n=5). Linearity is reported across dilution factors of 1:2, 1:4, 1:8, and 1:16 in serum, EDTA plasma, and heparin plasma.