Mouse Signal Transducer And Activator Of Transcription 1 ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of mouse STAT1 in tissue homogenates, cell lysates or other biological fluids. The assay employs a pre-coated microplate, a biotinylated anti-STAT1 detection antibody and an HRP-avidin conjugate, with a TMB substrate producing a colorimetric signal read at 450 nm. The manufacturer reports an assay range of 0.312–20 ng/ml and a sensitivity of 0.127 ng/ml, with the full protocol completed in 4 hours 30 minutes.

Validated for mouse reactivity, the kit is supplied with a lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution and adhesive plate sealers. Reported recovery values are provided for serum (80–102%, average 91%, n=5), EDTA plasma (81–100%, average 90%, n=5) and heparin plasma (80–89%, average 84%, n=5), along with linearity data across 1:2 to 1:16 dilutions for each sample type. Components should be stored at +4°C or −20°C as indicated on individual labels, and the kit should not be used past its expiration date.