Mouse Sideroflexin 1 ELISA Kit

This sandwich ELISA from Antibodies.com is designed for the quantitative in vitro determination of mouse SFXN1/TCC (Sideroflexin 1) in tissue homogenates and other biological fluids. The assay uses a pre-coated 96-well microplate format, runs in 4 hours 30 minutes, and is read colourimetrically at 450 nm on a standard microplate reader after TMB substrate development.

Reported performance includes an assay range of 0.156–10 ng/ml and a sensitivity of <0.059 ng/ml. Spike-and-recovery has been characterised across n=5 samples per matrix: serum (average 91%, range 80–102%), EDTA plasma (average 90%, range 81–100%) and heparin plasma (average 84%, range 80–89%). Linearity was assessed at 1:2, 1:4, 1:8 and 1:16 dilutions across the same sample types.

Kit components include a pre-coated microplate, lyophilised standard, Detection Solutions A and B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and adhesive plate sealers, with storage temperatures specified per item.