Mouse Serum Amyloid P Component ELISA Kit

A sandwich ELISA from Antibodies.com designed for the in vitro quantitative determination of mouse Serum Amyloid P Component in serum, plasma, tissue homogenates or other biological fluids. The assay is read on a colorimetric microplate reader at 450 nm and follows a sandwich enzyme immunoassay workflow, using a biotinylated detection antibody and HRP-avidin conjugate with a TMB substrate that yields a yellow product read against a standard curve.

With a sensitivity of <0.58 ng/ml and an assay range of 1.56-100 ng/ml, the kit supports quantitative measurement. Total assay time is 4h 30m. Average recovery has been validated in serum (n=5, 91%, range 80-102%), EDTA plasma (n=5, 90%, range 81-100%), and heparin plasma (n=5, 84%, range 80-89%). Linear dilution performance is reported across the same sample types at 1:2, 1:4, 1:8, and 1:16 dilutions.

Kit components should be stored at +4°C or -20°C as instructed and used before the expiration date.