Mouse Serotonin Transporter ELISA Kit

Manufactured by Antibodies.com, this Sandwich ELISA kit enables in vitro quantitative determination of mouse Serotonin Transporter in tissue homogenates, cell lysates, and other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with colorimetric detection read at 450 nm in a microplate reader.

According to the manufacturer, the assay range is 0.156–10 ng/ml with a sensitivity of <0.055 ng/ml, and total assay time is 4h 30m. Components should be stored at +4°C or -20°C as instructed and include a pre-coated microplate, lyophilized standard, Detection Solution A, Detection Solution B, Wash Buffer (30X), Sample Dilution Buffer, TMB Substrate, Stop Solution, and plate sealers.

Reported recovery values are: serum average 91% (range 80–102%, n=5), EDTA plasma average 90% (range 81–100%, n=5), and heparin plasma average 84% (range 80–89%, n=5). Dilution linearity at 1:2, 1:4, 1:8, and 1:16 dilutions (n=5 each) is reported as: serum 87–91%, 87–107%, 74–101%, 92–97%; EDTA plasma 90–105%, 84–101%, 90–101%, 79–108%; heparin plasma 84–95%, 92–105%, 82–105%, 89–91%.