Mouse SDC4 ELISA Kit

A 4-hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com, designed for the in vitro quantitative determination of mouse Syndecan 4 in serum, plasma, tissue homogenates, and other biological fluids. The assay uses a pre-coated microplate (12 x 8 well strips) with an immobilised capture antibody specific for Syndecan 4, followed by a biotinylated detection antibody and HRP-Streptavidin conjugate. TMB substrate is then added and the colour change is read at 450 nm to quantify the target.

The reported sensitivity is <0.188 ng/mL and the working range is 0.313–20 ng/mL. The kit is specific for Syndecan 4 with no reported cross-reactivity with other analogs or variants. Reactivity is mouse. Detection is colorimetric. Manufacturer-validated average sample recovery values are 94% for serum, 96% for EDTA plasma, and 96% for heparin plasma; serum and plasma samples are recommended to be diluted at least 1:2 with Sample Dilution Buffer prior to testing.

The product is classified under Assays and Kits. Synonyms associated with the target protein include Amphiglycan, Ryudocan core protein, SDC4, SYND4, and Syndecan-4.