Mouse Sclerostin ELISA Kit

This sandwich ELISA from Antibodies.com is designed for the in vitro quantitative determination of mouse Sclerostin in serum, plasma, tissue homogenates or other biological fluids. The kit employs a sandwich enzyme immunoassay technique using an antibody specific for Sclerostin pre-coated onto a 96-well microplate (12 x 8 well strips), with detection via a Biotinylated Anti-Sclerostin Antibody and HRP-Avidin conjugate, visualised with TMB substrate and read at 450 nm on a colorimetric microplate reader.

The stated sensitivity is <6.5 pg/ml and the assay range is 15.6-1000 pg/ml, with a total assay time of 4 hours 30 minutes. Kit components include a pre-coated microplate, lyophilised standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, to be stored at +4°C or -20°C as instructed. Reported average recovery ranges are 80-102% (serum), 81-100% (EDTA plasma) and 80-89% (heparin plasma), with dilution linearity assessed from 1:2 to 1:16 in the same matrices.