Mouse RXFP1 ELISA Kit

Antibodies.com offers this Mouse RXFP1 ELISA Kit as a 4-hour sandwich ELISA designed for the in vitro quantitative determination of mouse RXFP1 in serum, plasma, tissue homogenates, and other biological fluids. The assay uses a pre-coated microplate arranged in 12 x 8 well strips, with standards and samples binding to an immobilized capture antibody specific for RXFP1. A biotinylated detection antibody and HRP-Streptavidin conjugate, used with TMB substrate, enable colorimetric detection at 450 nm, where the intensity of the resulting yellow product is directly proportional to RXFP1 concentration.

The kit has a reported standard curve range of 15.625-1000 pg/mL and a sensitivity of 9.375 pg/mL. It is described as specific for RXFP1 with no reported cross-reactivity with other analogs or variants. Serum and plasma samples are recommended to be diluted at least 1:2 with Sample Dilution Buffer prior to testing, with validated sample dilutions noted for healthy serum and mouse brain tissue homogenate.

Validation data report average recovery values of 93% for serum (range 87-102%, n=10), 96% for EDTA plasma (range 91-101%, n=10), and 91% for heparin plasma (range 86-99%, n=10). The kit is shipped at +4°C, and components are stored at +4°C or -20°C as instructed in the Protocol Manual.