Mouse Retinol Binding Protein 4, Plasma ELISA Kit

This sandwich-format ELISA kit from Antibodies.com is intended for the in vitro quantitative determination of mouse Retinol Binding Protein 4 (RBP4) in tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips and is run on a colorimetric microplate reader. Following incubation, the captured analyte is detected with a biotinylated anti-RBP4 antibody and an HRP-avidin conjugate, with HRP enzymatic activity visualized using TMB substrate and quantified by reading absorbance at 450 nm.

The total assay time is 4 hours 30 minutes. The reported detection range is 3.12-200 ng/ml with a sensitivity of <1.15 ng/ml. Components should be stored between +4°C and -20°C as instructed and used prior to the indicated expiration date.