Mouse Receptor I For The Fc Region Of Immunoglobulin E ELISA Kit

From Antibodies.com, this sandwich ELISA kit is intended for the in vitro quantitative determination of mouse Fc epsilon RI (FCER1A) in tissue homogenates, cell lysates, or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips and a colourmetric detection format read on a microplate reader at 450 nm.

Reported performance figures are a sensitivity of <0.069 ng/ml and an assay range of 0.156–10 ng/ml, with a total assay time of 4 hours 30 minutes. Kit components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.

In the supplied validation data, average recovery was 91% in serum (n=5, range 80–102%), 90% in EDTA plasma (n=5, range 81–100%), and 84% in heparin plasma (n=5, range 80–89%), with corresponding dilution linearity ranges reported for 1:2, 1:4, 1:8, and 1:16 sample dilutions across these sample types.