Mouse R-Spondin 1 ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) kit is designed for the in vitro quantitative determination of mouse R-Spondin 1 (RSPO1) in tissue homogenates, cell lysates or other biological fluids. The assay uses a pre-coated 96-well microplate in a 12 x 8 well strip format, is read on a colorimetric microplate reader at 450 nm, and has a total assay time of 4h 30m. Sensitivity is stated as <5.8 pg/ml with an assay range of 15.6-1000 pg/ml.

Kit components include the pre-coated 96-well microplate, lyophilized standard (2 vials), Detection Solution A (120 µl), Detection Solution B (120 µl), 30X Wash Buffer (20 ml), Sample Dilution Buffer (45 ml), TMB Substrate (9 ml), Stop Solution (6 ml) and 5 adhesive plate sealers. Storage conditions vary by component between +4°C and -20°C, and kit components should not be used past the expiration date.

The manufacturer supplies recovery and dilution linearity data for serum, EDTA plasma and heparin plasma at dilutions of 1:2, 1:4, 1:8 and 1:16, and states the kit is suitable for cell lysates and tissue homogenates. The product is listed under the Antibodies.com Assays and Kits category and is reactive with mouse samples.