Mouse Protein Z ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (ELISA) is intended for the in vitro quantitative determination of mouse Protein Z in plasma. The format uses a pre-coated 96-well microplate, a biotinylated anti-Protein Z detection antibody, an HRP-avidin conjugate and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader.

Quoted performance figures from the manufacturer include a sensitivity of <6.5 pg/ml and a standard range of 15.6–1000 pg/ml, with a total assay time of 4 h 30 min. Reported recovery data (n=5 per matrix) are: serum average 91% (range 80%–102%), EDTA plasma average 90% (range 81%–100%) and heparin plasma average 84% (range 80%–89%). Linearity is provided across sample dilutions of 1:2, 1:4, 1:8 and 1:16 for serum, EDTA plasma and heparin plasma. Kit components should be stored at +4°C or –20°C as indicated in the product insert and the kit must not be used past its stated expiration date.