Mouse Protein Kinase B Alpha ELISA Kit

Designed for the in vitro quantitative determination of mouse AKT1, this sandwich ELISA from Antibodies.com is intended for use with tissue homogenates, cell lysates and other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips. Captured AKT1 is detected with a biotinylated anti-AKT1 antibody, followed by HRP-avidin conjugate and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader.

The kit reports a measuring range of 0.312–20 ng/ml and a sensitivity of <0.112 ng/ml, with a total assay time of 4 h 30 min. According to the manufacturer's specifications, sample recovery ranges from 80–102% in serum, 81–100% in EDTA plasma and 80–89% in heparin plasma. Kit components are stored at +4 °C or –20 °C as instructed and the kit is suitable for cell lysates and tissue homogenates.