Mouse Programmed Cell Death Protein 1 Ligand 1 ELISA Kit

This sandwich-format immunoassay kit from Antibodies.com enables in vitro quantitative measurement of mouse PD-L1 in serum, plasma, tissue homogenates and other biological fluids. The assay is built around a pre-coated 96-well microplate supplied as 12 × 8 well strips, and detection is read colorimetrically at 450 nm on a standard microplate reader. The full protocol can be completed in 4 hours 30 minutes.

Using the sandwich enzyme immunoassay principle, standards and samples are added to the wells where PD-L1 is captured by an immobilised antibody. After washing, a Biotinylated Anti-PD-L1 Antibody is added, followed by an HRP-Avidin conjugate. TMB substrate is then used to visualise the enzymatic reaction, producing a blue product that turns yellow upon addition of the acidic stop solution; the absorbance density is proportional to PD-L1 concentration and is calculated against the standard curve. The stated sensitivity is <0.058 ng/ml and the assay range is 0.156–10 ng/ml.

Kit components include the pre-coated 96-well plate, lyophilised standard (2 vials), Detection Solution A, Detection Solution B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and plate sealers, with storage at +4°C or −20°C as instructed and the kit not to be used past its expiration date. Reported mean recoveries are 91% for serum (range 80–102%), 91% for EDTA plasma (81–101%) and 84% for heparin plasma (80–89%), with dilution linearity data supplied at 1:2 to 1:16 for each of the three sample matrices.