Mouse Phosphatase And Tensin Homolog ELISA Kit

Antibodies.com supplies a sandwich ELISA kit for the in vitro quantitative determination of mouse PTEN (Phosphatase and Tensin Homolog) in tissue homogenates, cell lysates or other biological fluids. The assay uses a pre-coated 96-well microplate in a 12 x 8 well strip configuration, with colourmetric detection on a microplate reader measuring absorbance at 450nm, and a total assay time of 4h 30m.

Reported quantitative performance includes a product range of 0.156-10 ng/ml and a sensitivity of <0.061 ng/ml. Validation across serum, EDTA plasma and heparin plasma samples (n=5 each) gave average recovery values of 91%, 90% and 84%, with ranges of 80%-102%, 81%-100% and 80%-89% respectively. Dilution linearity was assessed at 1:2, 1:4, 1:8 and 1:16 for each sample matrix.

The kit is supplied with a pre-coated microplate, lyophilised standard, Detection Solution A, Detection Solution B, Wash Buffer (30X), Sample Dilution Buffer, TMB Substrate, Stop Solution and plate sealers. Components are stored between +4°C and -20°C as indicated, and the kit should not be used past its expiration date.