Mouse Parathyroid Hormone Related Protein ELISA Kit

From Antibodies.com, this sandwich ELISA is designed for the in vitro quantitative determination of mouse PTHLH in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The assay employs a pre-coated microplate with an antibody specific for PTHLH, a biotinylated anti-PTHLH detection antibody, an HRP-avidin conjugate, and a TMB substrate, with absorbance read at 450 nm in a microplate reader. It is reactive with mouse samples.

The reported measurement range is 15.6-1000 pg/ml, with a sensitivity of <5.6 pg/ml. The standard assay time is 4h 30m. Reported average recoveries across an evaluated sample set (n=5) are 95% for serum (range 90%-101%), 95% for EDTA plasma (range 91%-99%), and 94% for heparin plasma (range 90%-99%). Dilution linearity at 1:2, 1:4, 1:8, and 1:16 dilutions has been demonstrated across serum, EDTA plasma, and heparin plasma samples (n=5). Synonyms listed for the target include parathyroid hormone-like protein, PLP, PTH-rP, and PTHrP.