Mouse Osteoglycin ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the quantitative in vitro determination of mouse osteoglycin in tissue homogenates, cell lysates and other biological fluids. The assay uses a pre-coated 96-well microplate and a biotinylated detection antibody with HRP-avidin conjugate and TMB substrate, with absorbance read at 450 nm. The reported sensitivity is 0.066 ng/ml and the assay range is 0.156–10 ng/ml, with a total assay time of 4h 30m.

Manufacturer validation data report recovery averages of 91% in serum (range 80–102%, n=5), 90% in EDTA plasma (range 81–100%, n=5) and 84% in heparin plasma (range 80–89%, n=5), with linearity tested across sample dilutions of 1:2 to 1:16. Kit components include pre-coated plate strips, lyophilized standard, detection reagents A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, with storage at +4°C or -20°C as indicated.

For research use only; not intended for diagnostic or therapeutic applications.