Mouse Neuronal Pentraxin I ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com for the in vitro quantitative determination of mouse Neuronal Pentraxin I (NP-I) in tissue homogenates or other biological fluids. The assay employs a pre-coated microplate and is read on a colorimetric microplate reader at 450 nm, with a total assay time of 4h 30m.

Quantitative performance specifications include a measuring range of 0.156-10 ng/ml and a sensitivity of <0.064 ng/ml. Reported sample validation values across n=5 determinations are: serum 80%-102% (average 91%), EDTA plasma 81%-100% (average 90%), and heparin plasma 80%-89% (average 84%). Dilution linearity across 1:2, 1:4, 1:8 and 1:16 dilutions is reported as: serum 87-91%, 87-107%, 74-101%, 92-97%; EDTA plasma 90-105%, 84-101%, 90-101%, 79-108%; heparin plasma 84-95%, 92-105%, 82-105%, 89-91%.

Components supplied include a pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer (30X), sample dilution buffer, TMB substrate, stop solution and plate sealers. Kit components should be stored at +4°C or -20°C as instructed.