Mouse MMP-9 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com designed for the in vitro quantitative determination of mouse MMP9 in serum, plasma, tissue homogenates, and other biological fluids. The kit is reported as specific for MMP9 with no cross-reactivity with other analogs or variants. Detection is colorimetric, with absorbance read at 450 nm following HRP-Streptavidin and TMB substrate development, and the assay is completed in approximately four hours on a pre-coated microplate format.

The stated product range is 78.125–5000 pg/mL with a sensitivity of <46.875 pg/mL. Manufacturer recovery data report mean values of 97% for serum, 97% for EDTA plasma, and 99% for heparin plasma (each n=10), with linearity demonstrated across 1:2, 1:4 and 1:8 dilutions. Serum and plasma samples should be diluted at least 1:2 with Sample Dilution Buffer prior to testing. Kit components are shipped at +4°C and stored at +4°C according to the protocol manual, and include a pre-coated plate, lyophilized standard, sample and antibody dilution buffers, biotinylated detection antibody, HRP-Streptavidin conjugate, SABC dilution buffer, TMB substrate, stop solution, wash buffer and plate sealers.