Mouse Microseminoprotein Beta ELISA Kit

This sandwich ELISA kit from Antibodies.com is intended for the in vitro quantitative determination of Prostate Secretory Protein/PSP (Microseminoprotein Beta) in mouse serum, plasma or other biological fluids. The product belongs to the Assays and Kits category.

The assay uses the sandwich enzyme immunoassay technique on a pre-coated microtiter plate. Standards and samples are incubated with a biotinylated anti-Prostate Secretory Protein/PSP antibody, followed by an HRP-avidin conjugate. A TMB substrate is added to generate a colour change, which is read at 450 nm on a microplate reader; target concentration is then derived from a standard curve. Reported performance is an assay range of 0.156–10 ng/ml and a sensitivity of <0.072 ng/ml, with a total assay time of 4h 30m. Kit components are stored at +4°C or −20°C as instructed.

Documentation cites spike-and-recovery averages of 91% in serum (80–102%, n=5), 90% in EDTA plasma (81–100%, n=5) and 84% in heparin plasma (80–89%, n=5), together with linearity-of-dilution data across 1:2, 1:4, 1:8 and 1:16 dilutions for each matrix.