Mouse Melanoma Inhibitory Activity Protein 1 ELISA Kit

Antibodies.com supplies a sandwich ELISA kit for the in vitro quantitative determination of mouse Melanoma Inhibitory Activity Protein 1 in serum, plasma, tissue homogenates and other biological fluids. The assay is run on a pre-coated 96-well microplate (12 x 8 well strips) and uses a biotinylated detection antibody with HRP-avidin and TMB substrate; absorbance is read at 450 nm on a colorimetric microplate reader.

The working range is 0.781–50 ng/ml with a sensitivity of 0.25 ng/ml, and the full protocol takes approximately 4 hours 30 minutes. Reported recovery in spiked samples is 80–102% for serum, 81–100% for EDTA plasma and 80–89% for heparin plasma. Kit components should be stored at +4°C or -20°C as instructed.