Mouse Macrophage Inflammatory Protein 3 Beta ELISA Kit

Antibodies.com provides a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) for the in vitro quantitative determination of mouse MIP-3 beta/CCL19 in serum, plasma or other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips), a biotinylated anti-MIP-3 beta/CCL19 detection antibody, HRP-avidin conjugate, and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader.

Quoted performance specifications include an assay range of 15.6-1000 pg/ml and a sensitivity of 5.9 pg/ml, with a total assay time of 4 hours 30 minutes. Recovery across five samples per matrix is reported at an average of 91% in serum (range 83-100%), 94% in EDTA plasma (range 91-98%), and 94% in heparin plasma (range 90-99%). Linear dilution behaviour is also reported for 1:2, 1:4, 1:8 and 1:16 dilutions in each matrix.

Kit contents include the pre-coated microplate strips, lyophilised standard, Detection Solutions A and B, Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution, and plate sealers. Components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.