Mouse Low Density Lipoprotein Receptor Adaptor Protein 1 ELISA Kit

Antibodies.com supplies a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) intended for the in vitro quantitative determination of mouse ARH (Low Density Lipoprotein Receptor Adaptor Protein 1) in tissue homogenates or other biological fluids. Reactive with mouse samples, the kit employs a pre-coated microplate arranged as 12 x 8 well strips and is read on a colorimetric microplate reader at 450 nm.

The manufacturer reports a sensitivity of <0.067 ng/ml and an assay range of 0.156–10 ng/ml, with a total assay time of approximately 4 hours 30 minutes. In the sandwich immunoassay workflow, an antibody specific for ARH is pre-coated onto the plate to capture ARH from standards and samples; a biotinylated anti-ARH antibody then binds the captured analyte, and an HRP-Avidin conjugate together with TMB substrate is used to generate a coloured product that changes from blue to yellow upon addition of acidic stop solution. The concentration of ARH is calculated from the O.D. absorbance at 450 nm against the standard curve.

Reagent components are stored at +4°C or -20°C as instructed, and the product is for research use only.