Mouse LIPE ELISA Kit

From Antibodies.com, the Mouse Hormone Sensitive Lipase (HSL) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (sELISA) intended for the in vitro quantitative determination of mouse Hormone Sensitive Lipase/HSL in serum, plasma, tissue homogenates, and other biological fluids. The manufacturer states no reported cross-reactivity with other analogs or variants, and the assay is completed in approximately 4 hours. Quantification is by colorimetric detection measured at 450 nm using a pre-coated microplate platform.

The stated analytical range is 15.625–1000 pg/mL with a sensitivity of 9.375 pg/mL. Internal recovery evaluation (n=10) reported an average of 98% for serum (range 94–102%), 99% for EDTA plasma (93–103%), and 99% for heparin plasma (89–104%). Validated dilutions for healthy serum and plasma are 1:2–1:5 with the provided Sample Dilution Buffer, and all samples should be diluted at least 1:2 prior to testing. The kit is shipped at +4°C, with selected components also storable at −20°C as directed in the protocol manual; freeze/thaw cycles and use past the expiration date should be avoided.